088 Microprojectile Bombardment-mediated Transformation of Rhododendron `Catawbiense Album' L.

Transformation of Filamentous Fungi by Microprojectile Bombardment

The vast majority of transformation protocols for filamentous fungi are based on permeabilizing cell membranes with polyethylene glycol (PEG) or electroporation. Both methods typically require preparing protoplasts or osmotically sensitive cells prior to transformation (Herzog et al., 1996; Goldman et al., 1990; a detailed review of fungal transformation systems is given by Lemke and Peng, 1995...

Genetic Transformation of Immature Sorghum Inflorescence via Microprojectile Bombardment

Transformation of Aspergillus nidulans by microprojectile bombardment on intact conidia.

This paper describes transformation of intact conidia of Aspergillus nidulans, auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/microgram of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integrat...

Transgenic sorghum plants via microprojectile bombardment.

Transgenic sorghum plants have been obtained after microprojectile bombardment of immature zygotic embryos of a drought-resistant sorghum cultivar, P898012. DNA delivery parameters were optimized based on transient expression of R and C1 maize anthocyanin regulatory elements in scutellar cells. The protocol for obtaining transgenic plants consists of the delivery of the bar gene to immature zyg...

Agrobacterium Mediated Transformation of Maize (Zea mays L.)

Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...